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Image Search Results
Journal: bioRxiv
Article Title: High efficiency organoid-derived cyst cultures as a drug discovery platform for polycystic kidney disease
doi: 10.1101/2025.02.16.638545
Figure Lengend Snippet: A . Schematic of the protocol. B . Day (d) 12 nephron organoids differentiated from wildtype iPSCs. C . Enzymatic digestion of whole nephron organoids into a single cell suspension, marking d0 of UB differentiation. D . Development of UB organoids over 15 days. E . qPCR analysis comparing d12 nephron organoids and d7 UB organoids for nephron and UB marker gene expression. ** p≤ 0.01; *** p≤ 0.001; **** p≤ 0.0001 (Unpaired t -test). F . Immunohistological characterization of UB organoids showing expression of distal tubule (CDH1, PAX2, GATA3) and UB markers (RET, KRT8). Nuclear counterstain, Hoechst. Scale bars: 400 µm (B-D); 100 µm (F).
Article Snippet: Antibodies used were: PAX2 (BioLegend 901001), CDH1 (BD Biosciences 610181),
Techniques: Suspension, Marker, Gene Expression, Expressing
Journal: bioRxiv
Article Title: High efficiency organoid-derived cyst cultures as a drug discovery platform for polycystic kidney disease
doi: 10.1101/2025.02.16.638545
Figure Lengend Snippet: A . Development of PKD2 -/- UB organoids over 25 days. Arrow shows an early cyst on d7. B . Quantification of cyst development in wildtype and PKD2 -/- UB organoids. C . PKD2 -/- cyst diameters increase over time. D . Example of a high efficiency cyst culture at d17. E . Upregulation of ADPKD marker genes in PKD2 -/- compared to wildtype UB organoids. ** p≤ 0.01; *** p≤ 0.001; **** p≤ 0.0001 (Unpaired t -test). F . Immunohistological analysis of PKD2 -/- UB organoids, showing expression of distal tubule and UB markers CDH1, PAX2, RET and GATA3 in the cystic epithelium. Nuclear counterstain, Hoechst. Scale bars: 400 µm (A); 1000 µm (D); 100 µm (F).
Article Snippet: Antibodies used were: PAX2 (BioLegend 901001), CDH1 (BD Biosciences 610181),
Techniques: Marker, Expressing